शैडोइंग अभ्यास: CRISPR-Cas9 Genome Editing Technology - YouTube के साथ अंग्रेजी बोलना सीखें

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We’ve examined a handful of biotechnology concepts  in previous tutorials, but now it’s time to introduce what is undoubtedly the most promising  technique in biotechnology of the past decade. The CRISPR-Cas9 system represents genome editing  technology that has revolutionized molecular biology, due to its precise and site-specific gene  editing capabilities, which essentially allow for an unprecedented level of control in manipulating  the genetic information of a living organism.
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We’ve examined a handful of biotechnology concepts  in previous tutorials, but now it’s time to introduce what is undoubtedly the most promising  technique in biotechnology of the past decade. The CRISPR-Cas9 system represents genome editing  technology that has revolutionized molecular biology, due to its precise and site-specific gene  editing capabilities, which essentially allow for an unprecedented level of control in manipulating  the genetic information of a living organism.
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How does this work mechanistically, and what are  its applications? Let’s get a closer look now, starting with some historical context. In 1987, Atsuo Nakata and his team of researchers from the Osaka University in  Japan first reported the presence of Clustered Regularly Interspaced Short Palindromic Repeats,  abbreviated as CRISPR, in the Escherichia coli genome. These refer to short, repeated sequences  of DNA nucleotides found within the genome of prokaryotes. These sequences are the same  when read from 5' to 3' on one strand of DNA and from 5' to 3' on the complementary strand, and  are therefore described as palindromic repeats, just the way that we refer to words like racecar  or kayak as being palindromes, because they are the same whether read forwards or backwards. This  was further reported in both Gram-positive and Gram-negative bacteria, along with archaea,  leading to the obvious question regarding the relevance of CRISPR to these organisms,  which drove research for some time. Later on, in the mid 2000s, the functionality and importance  of CRISPR was first realized in prokaryotes. As it turns out, the CRISPR system is a key component  of their adaptive immunity, which protects these prokaryotes from attack by viral DNA,  bacteriophages, and plasmids. That’s right, it may seem incredible, but even unicellular bacteria  have a very basic immune system. Recall from our studies in the immunology series that adaptive  immunity refers to the immunity that an organism acquires after exposure to an antigen, either  from a pathogen or vaccination. Vaccination, for example, results in a form of adaptive  immunity in humans, since the body is exposed to antigens, and forms antibodies in response, which  contribute to the development of the immunity.
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The way this works for bacteria is as follows. The  unique sequences that are nestled in between the palindromic repeats, which are called spacers, are  bits of DNA that are foreign, and do not belong to the bacterium, but instead originate from mobile  genetic elements, or MGEs, such as bacteriophages, transposons, or plasmids that have  previously infected the prokaryote.
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This was revealed by sequencing the spacers found  in the CRISPR system, which led to the hypothesis that this could be a defense mechanism employed  by bacteria to recognize foreign DNA elements.
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During a viral infection, bacteria acquire  a small piece of the foreign viral DNA, and integrate it into the CRISPR locus to  generate CRISPR arrays. These consist of duplicate sequences, which are the palindromic  repeats belonging to the bacterial genome, flanked by variable sequences, or spacers, which  again are from the foreign genetic elements.
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In this way, bacteria retain a memory,  so to speak, of a past infection.
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So although it was initially revealed as a  genomic component of bacteria and archaea, CRISPR has inspired a method of genome editing  that can be applied to various eukaryotic species. But before we get there, we first have to  understand the function of CRISPR in prokaryotes, because understanding the mechanism of its natural  function will be necessary in order to understand the way it is exploited to achieve genome editing  capabilities in humans and other organisms.
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Let’s take a look at a particular Streptococcus  bacterium which is being attacked by a bacteriophage. Once the viral DNA is injected into  the cell, a section of it can be incorporated into the bacterial genome, and as we mentioned, it  will be inserted between the repeated palindromic sequences. This will now be called a spacer.  So here we can see three different spacers, potentially from three different viruses,  sandwiched in between the repeated palindromic sequences. Now we have what is called a  CRISPR array. This CRISPR array can undergo transcription, to form CRISPR RNA, abbreviated  as crRNA, although this longer strand is called pre-crRNA. Then the protein Cas9 gets involved.  Cas refers to CRISPR-associated nuclease protein, and as we know, nucleases are enzymes  that are capable of cleaving DNA at specific nucleotide linkages, kind of  like a pair of scissors. In particular, Cas9 is one of the nucleases found in Streptococcus  pyogenes, which is one of the most extensively researched and characterized CRISPR-associated  nuclease proteins, so this is the one we will be looking at here inside this bacterium. Now along with Cas9, there are also molecules of tracrRNA. These have sections  that are complementary to and therefore can anneal to the palindromic repeats. So for  each spacer and palindromic repeat, we end up with a complex consisting of that segment of  pre-crRNA, a tracrRNA, and a Cas9 protein.
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Then another enzyme called ribonuclease three,  or RNase III, will cleave the strand in between these complexes, leaving us with individual crRNA  complexes which we can call effector complexes.
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With these effector complexes formed, the cell  is now ready to defend against the invader whose genome produced that crRNA. If this  complex encounters a section of viral DNA that has a sequence which is complementary to this  crRNA, the nuclease enzyme will coordinate, and if it recognizes a short sequence unique to the  viral genome called a protospacer adjacent motif, or PAM, then it will snip both strands of the  DNA, just a few base pairs upstream from the PAM.
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In doing so, it will neutralize the  virus, because its genome can no longer be transcribed properly to create more  viral particles, so infection is impossible.
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So that gives us a reasonable understanding of  how CRISPR is employed by prokaryotic organisms as a natural defense. Now it’s time to understand  how this phenomenon came to serve as the basis for biotechnological application. This begins in  2012, when Jennifer Doudna, a molecular biologist from the University of California, Berkeley  along with French microbiologist Emmanuelle Charpentier, were the first to propose  that the bacterial CRISPR-Cas9 system could be used as a programmable toolkit for  genome editing in humans and other animal species, and they eventually received the Nobel  prize in chemistry for their work, in 2020.
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So how can genome editing be achieved using this  method? The first thing we need to understand is that in bacteria, the crRNA and tracrRNA are  separate molecular entities. The first major breakthrough arrived when it was realized that  the roles of these molecules could be combined into a single molecule by fusing them together  with a linker to generate something called single guide RNA, or sgRNA, which  can be synthesized in the lab.
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If the sgRNA complexes with a Cas9 protein, this  two-component system will be able to cleave DNA just as the three-component system does in  bacteria. What this means was that it was then possible to determine any sequence of about 20  base pairs as a target for editing, and all that has to be done is to synthesize the appropriate  sgRNA with the complementary sequence, and insert that into a cell along with the Cas9 protein which  has been sourced from Streptococcus pyogenes.
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The complex will form, read the DNA until it  finds the appropriate sequence along with a PAM sequence, binding will occur, and DNA will  be cleaved at precisely the desired location.
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Cas9 has two domains, and each one  will snip one of the DNA strands.
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After the incision is made, the natural DNA  repair mechanism is enacted for the target DNA.
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The cleaved dsDNA can undergo repair via two  routes. Either by homology-directed repair, abbreviated as HDR, or by non-homologous end  joining, abbreviated as NHEJ. The NHEJ pathway repairs double-strand breaks in DNA by directly  ligating without the need for a homologous template, which means a DNA strand with similar  sequence that can act as a template. The NHEJ mechanism can also introduce insertion or deletion  of specific sequences at the joining ends, thus creating what are referred to as indels.  Indels are DNA strands with either an insertion or deletion of nucleotide sequences. Thus, NHEJ  produces DNA strands with non-uniformity in size.
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The other route of repair, the HDR pathway, is  commonly found in bacterial and archaeal cells, while the NHEJ pathway we just discussed is more  common in a eukaryotic domain. The HDR process, although more complex than NHEJ, uses a homologous  DNA template. The homologous DNA template has homology to the adjacent sequences surrounding the  site of cleavage to incorporate new DNA fragments.
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The template guides the repair process, and  lowers the possibility of errors. Since there is no insertion or deletion of nucleotide sequences,  the HDR pathway maintains uniformity in the size of the resulting dsDNA, unlike NHEJ. So that covers the mechanism of CRISPR genome editing technology. Now we move on  to the potential applications, which have only expanded ever since Doudna and Charpentier  suggested the possibility of using CRISPR for genome editing in humans and other animals. The  potential scope of application of CRISPR is vast, and includes its use as a genetic screen  to identify genes in different cells.
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One of the most prominent applications is in  cancer immunotherapy. In this practice, immune T cells, which are a type of white blood cell that  fights against a disease, are genetically modified using CRISPR technology. Specifically, these  T cells are extracted from the patient’s body and modified to make them more specialized in  recognizing cancer cells and killing them once they are reintroduced into the patient’s body. Similarly, CRISPR has also found its application in therapeutic management of acquired  immunodeficiency syndrome, or AIDS, which is caused by human immunodeficiency virus, also known  as HIV, as we covered in the microbiology series.
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Conventional anti-retroviral therapies are  capable of suppressing viral replication. But once the virus gets converted to its proviral  form, conventional therapies are ineffective in targeting the virus. The provirus resides  within the immune cells and continues to make copies of itself using the immune cell machinery,  and the immune cells fail to target the proviral latent reservoir which presents the risk  of viral rebound or relapse of the disease.
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Other than cancer and AIDS, CRISPR has also found  immense application in developing assays to detect SARS-CoV-2 infection, the cause  of the current global pandemic.
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Although genome editing of human embryos  and their implantation into a human womb, as well as genetic editing of somatic cells, have  wide ethical concerns and potential risks, CRISPR has the promise to cure various diseases and  prevent the inheritance of gene-linked diseases.
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Additionally, genome editing in  plants using CRISPR technology introduces the possibility of making  plants resistant to certain diseases, improving their phenotype or observable  characteristics, incorporating certain specific traits, improving crop yield, and so  forth. With so many invigorating possibilities for this exciting new technology, it will be  fascinating to see which of these major diseases and issues will be solved first, signaling  the dawn of a new era in molecular biology.
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इस वीडियो में हमें CRISPR-Cas9 जीन संपादन तकनीक के बारे में जानकारी मिलती है, जो बायोटेक्नोलॉजी के क्षेत्र में एक महत्वपूर्ण प्रगति का प्रतिनिधित्व करती है। वीडियो में वर्णित विशेष तकनीक ने आणविक जीवविज्ञान में क्रांति ला दी है, जो जीवों के आनुवंशिक जानकारी को सटीक और स्थान-विशिष्ट तरीके से संपादित करने की क्षमता प्रदान करती है। हमें यह भी बताया गया कि कैसे 1987 में जापान के ओसाका विश्वविद्यालय के शोधकर्ताओं ने CRISPR के अस्तित्व की खोज की और इसके कार्यप्रणाली को समझने के लिए एक ऐतिहासिक पृष्ठभूमि पेश की गई है।

दैनिक संचार के लिए शीर्ष 5 वाक्य

  • CRISPR-Cas9 तकनीक ने विज्ञान के क्षेत्र में एक नया आयाम जोड़ा है।
  • यह प्रणाली जीवन के आनुवंशिक सूचना को संपादित करने के अद unprecedented स्तर का नियंत्रण प्रदान करती है।
  • बैक्टीरिया में CRISPR उनकी निहित प्रतिरक्षा का एक महत्वपूर्ण घटक है।
  • यह तकनीक कैन्सर और एड्स जैसे रोगों के इलाज में क्रांतिकारी साबित हो रही है।
  • CRISPR ने पौधों के जीन संपादन में भी महत्वपूर्ण भूमिका अदा की है।

चरण-दर-चरण छायांकन गाइड

CRISPR-Cas9 जैसे तकनीकी विषयों को समझने में कठिनाई हो सकती है, लेकिन आप shadowspeak तकनीक का उपयोग करके अपनी समझ को बेहतर कर सकते हैं। यहाँ कुछ सुझाव दिए गए हैं:

  • वीडियो को बार-बार सुनें: वीडियो को बार-बार सुनने से आपको मूल विचारों और शब्दों की आदान-प्रदान की आदत बनानी होगी।
  • सुनें और बोलें: वीडियो के संवाद का अनुसरण करें और फिर से उसी संवाद को बोलें, इससे आपके अंग्रेजी उच्चारण में सुधार होगा।
  • प्रमुख शब्दों पर ध्यान दें: उन वाक्यों और शब्दों पर ध्यान दें जो आपको वीडियो में बार-बार सुनाई देते हैं, इससे उनकी सही उच्चारण सीखने में मदद मिलेगी।
  • स्पष्टता पर काम करें: हर बार जब आप दोहराते हैं, तो उच्चारण और स्पष्टता पर ध्यान दें, यह आपके संवाद कौशल को बढ़ाने में सहायक होगा।
  • अर्थ समझें: केवल सुनना ही नहीं, बल्कि यह भी समझें कि जो शब्द आप बोल रहे हैं उनका अर्थ क्या है। इससे आपकी संवाद करने की क्षमता में इजाफा होगा।

इस तरह, यूट्यूब से अंग्रेजी सीखें इस तकनीक के मदद से आपके संवाद कौशल में सुधार होगा। नियमित अभ्यास से आप अंग्रेजी में अधिक आत्मविश्वास के साथ संवाद कर सकेंगे।

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