Shadowing-Übung: PCR (Polymerase Chain Reaction) - Englisch Sprechen Lernen mit Video

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There are some pieces of technology you really develop an appreciation for.

Maybe it's your phone, your laptop, your drawing tablet, but for me, it is the copy machine.

Oh, the copy machine.

I know it's not possible, but I could promise you that whenever the copy machine was aware of my presence, that's when it decided to malfunction,

especially if it knew I was short on time.

And of course, when it jams, the fancy copy machines will even tell you what is wrong and how to fix it,

but my experience with trying to follow the instructions of fixing the copy machine makes it worse, and then by that point there's a line,

and you feel your heart pounding, and you're embarrassed because now you've jammed it for everyone.

Yeah, so while I've developed an appreciation and respect for the copy machine, I've definitely tried to limit my use of it over the years.

Saves paper anyway.

You may be wondering why is she spending so much time on this?

Well I want to call your attention to a technology, a biotechnology, that works almost like an amazing and fancy copy machine.

Except not for something on paper.

No, it's for DNA.

This biotechnology is PCR.

Polymerase Chain Reaction, or PCR for short.

It provides a way to make more copies of a portion of DNA.

Lots and lots and lots of DNA copies, and this technology does NOT need to happen in a cell either.

In fact, it can happen in a test tube.

And so you may wonder, number one, how does PCR work?

And number two, why?

Why make more copies of some specific portion of DNA?

So let's briefly answer those two questions.

To answer the first question, the how does this work question,

let's talk about what we need first before we get to the steps of how it works.

We need the DNA portion that we want to make copies of, we need some kind of buffer to put it in,

and then we need things that would be necessary to make more copies of the DNA.

So think about what that might mean from our DNA replication video.

We'll need primers.

Recall that primers help DNA polymerase, a building enzyme, know where to go to start its building.

We need DNA polymerase, the building enzyme.

Fun fact, the DNA polymerase used is often a heat -resistant type of DNA polymerase as PCR uses heat.

Typically, the polymerase chosen for the job is tach polymerase, a type of of heat -resistant DNA polymerase.

Tac polymerase is originally from a type of bacteria that can handle and live in really high temperatures in nature, in hot springs.

Pretty cool.

We also need DNA nucleotides for the DNA polymerase to build with.

So we can look at the PCR sequence in three major steps.

We'll illustrate with one double -stranded DNA molecule here, and let's assume this is what we want replicated.

Step 1.

Denaturation.

You've heard that word denature before, right, when we were talking about enzymes?

One way we had talked about denaturing enzymes was using heat.

And heat is what we will use in this step.

This step involves the addition of heat needed to separate the two strands of the DNA molecule.

Step 2.

Annealing.

This word means something a little different in biology.

Basically this is when the two DNA strands

that now have been separated by that heat are going to be cooled and be joined by the primers.

The temperature for this step should allow the primers to bind to the specific segment of DNA that you want to amplify,

which means make copies of.

Step 3.

DNA synthesis.

Remember, synthesis means to make something.

With DNA synthesis, we're going to make more copies of DNA, and to do so, DNA polymerase will begin to work on both of these strands

and it will use the DNA nucleotides as its building material to amplify the DNA.

We should note that the temperature at this step may be a little warmer than the previous step.

It needs to be a temperature that is ideal for the specific DNA polymerase used.

Now after finishing one cycle of this, you have two double -stranded DNA molecules, right?

Similar to how it would be in DNA replication within a cell.

But you can repeat this now, except this time you now have two double -stranded DNA molecules to start with.

So you repeat with the denaturation, annealing, and DNA synthesis steps, now you have four double -stranded DNA molecules.

You repeat the steps again, now you have eight.

And if the process is automated by a machine, which, why yes, they do have in science catalogs, you can actually do this fairly quickly.

Which brings you to, why?

Why do this?

Well, any technology that needs copies of a portion of DNA could find PCR useful, but But we'll just mention two examples in our limited time.

We mentioned DNA fingerprinting in our gel electrophoresis video, and we mentioned that DNA fingerprinting can be part of a crime scene investigation.

Well, in order to have enough copies of DNA samples to run in gel electrophoresis to analyze,

PCR can be performed to make copies of the of the fragments of DNA that are found at a crime scene.

Another example?

Diagnosis of a disease, such as one caused by a virus.

For an especially relevant example, I can mention one of the testing types done for the virus that causes COVID -19.

COVID -19 is a pandemic that we're experiencing right now in 2020.

And the virus that causes COVID -19 is called SARS -CoV -2.

You might have heard of one test type that uses a sample from a nose or throat swab in a PCR test,

but to be more specific, this test for this virus is a real -time reverse transcription PCR test.

The reason it has this fancier reverse transcription in there is

because this virus uses RNA as its genetic material instead of DNA,

and you have to use an enzyme called reverse transcriptase to convert the RNA into DNA.

So before we can do the regular PCR steps we've mentioned, we must convert isolated and purified RNA into DNA.

A specific primer is added that will bind to an area of viral RNA,

and then reverse transcriptase is used to convert viral RNA into cDNA .

Using specific primers and the TAC DNA polymerase,

the cDNA can be copied over and over each cycle in the familiar steps we've been mentioning of PCR.

See the goal is you need enough copies of the viral cDNA in order for it to be detectable.

The idea being if it's a positive result, you have the selective primers binding and you have the TAC DNA polymerase making more

and more copies of the viral cDNA each cycle.

In addition, specific fluorescent probes are also used for identification.

A certain level is needed for identification of a positive result.

And if the virus's genetic material is not present in the sample, then primers wouldn't bind, and there would be no cDNA copies produced.

If you'd like to learn more about this test as far as its limitations

and also its complexity as we're being pretty general here, we've included some further reading suggestions in the video details.

So those were just two examples of how PCR can be used,

but there are many more examples in our suggested further reading links in the video description.

Overall, PCR is such a useful and fascinating technology that will likely remain indispensable for future uses.

It's a rare day when I pull out that word, indispensable.

Well, that's it for the Amoeba Sisters, and we remind you to stay curious.

I'll see you next time.

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Wortschatz und Sprechhinweise zu dieser Lektion

Diese Wörter kommen am häufigsten vor: Dna, Pcr, polymerase, step, copies. Dieses Video enthält 83 Sätze und 1299 Wörter zum Nachsprechen. Der gesprochene Teil dauert 7:30. Der Sprecher spricht in natürlichem Tempo, etwa 173 Wörter pro Minute, ähnlich wie im Alltagsgespräch. Nur 81 % der Wörter gehören zu den 3.000 häufigsten im Englischen, der Wortschatz ist also anspruchsvoll.

Wichtiger Wortschatz in diesem Video

15 lernenswerte Wörter aus dem Video, mit Aussprache und Bedeutung:

Wort	Aussprache	Bedeutung
strand Substantiv	/ˈstɹænd/	Strang
virus Substantiv	/ˈvaɪ.ɹəs/	Virus, virus
enzyme Substantiv	/ˈɛn.zaɪm/	Enzym
molecule Substantiv	/ˈmɑ.lɪ.kjul/	Molekül
reverse Substantiv	/ɹɪˈvɝs/	Gegenteil
viral Adjektiv	/ˈvaɪɹəl/	Virus-, viral
synthesis Substantiv	/ˈsɪnθəsɪs/	Synthese
fancy Substantiv	/ˈfæn.si/	Vorstellungskraft, Einbildungskraft
convert Verb	/kənˈvɝt/	konvertieren, umwandeln
genetic Adjektiv	/d͡ʒəˈnɛt.ɪk/	genetisch
identification Substantiv	/aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/	Identifizierung
jam Substantiv	/ˈd͡ʒæm/	Marmelade, Konfitüre
appreciation Substantiv	/əˌpɹiː.ʃiˈeɪ.ʃən/	Anerkennung, Würdigung
resistant Adjektiv	/ɹəˈzɪstənt/	resistent
curious Adjektiv	/ˈkjʊɹ.i.əs/	neugierig

Sätze zum Wiederholen

Kurze, vollständige Sätze aus dem Video, die du im Alltag wiederverwenden kannst:

You’ve heard that word “denature” before, right?
When we were talking about enzymes?
Which brings you to…why?

Grammatik in diesem Video

Die Strukturen, die der Sprecher am häufigsten verwendet, mit den genauen Worten aus dem Video:

Struktur	Im Video
Passiv be + Partizip Perfekt – wichtig ist, was geschieht, nicht wer es tut	have been separated · is automated · can be performed
Present Perfect have/has + Partizip Perfekt – eine vergangene Handlung, die jetzt noch wichtig ist	you've jammed · I've developed · You've heard

Aussprache, auf die du achten solltest

Der Sprecher verwendet 16 Kurzformen und abgeschwächte Formen, zum Beispiel we'll, we're, we've. Sprich sie in der kurzen Form, so wie du sie hörst.

Die „th“-Laute: synthesis /ˈsɪnθəsɪs/, throat /ˈθɹoʊt/
Die Laute „sh“ und „zh“: identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, appreciation /əˌpɹiː.ʃiˈeɪ.ʃən/, transcription /tɹænˈskɹɪpʃən/, suggestion /səˈd͡ʒɛst.ʃən/, instruction /ɪnˈstɹʌkʃən/
Lange Wörter – auf die Betonung achten: identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, appreciation /əˌpɹiː.ʃiˈeɪ.ʃən/, biology /baɪˈɑ.lə.d͡ʒi/, complexity /kəmˈplɛk.sɪ.ti/, limitation /lɪmɪˈteɪʃən/

Laute, die Deutschsprachigen schwerfallen:

/w/ — mit runden Lippen, nicht wie das deutsche „w“ (/v/): sequence /ˈsiː.kwəns/, swab /swɒb/
Stimmhafter Auslaut — /b/, /d/, /g/, /z/, /v/ am Wortende nicht verhärten: strand /ˈstɹænd/, bind /ˈbaɪ̯nd/, remind /ɹəˈmaɪnd/, tube /tuːb/, pound /ˈpaʊ̯nd/
/æ/ — offener als „ä“: strand /ˈstɹænd/, fancy /ˈfæn.si/, jam /ˈd͡ʒæm/, transcription /tɹænˈskɹɪpʃən/, laptop /ˈlæp.tɑp/

So übst du mit diesem Video

Höre dir das ganze Video einmal an, ohne zu sprechen, und notiere die Wörter, die du nicht kennst.
Beginne mit 0,75-facher Geschwindigkeit, sprich Satz für Satz nach und wechsle zur normalen Geschwindigkeit, sobald es leichtfällt.
Nimm dich auf und vergleiche mit dem Original; achte dabei auf Wörter wie strand, virus, enzyme.

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Shadowing ist eine wissenschaftlich fundierte Sprachlerntechnik, die ursprünglich für die professionelle Dolmetscherausbildung entwickelt und durch den Polyglotten Dr. Alexander Arguelles populär gemacht wurde. Die Methode ist einfach aber wirkungsvoll: Du hörst englisches Audio von Muttersprachlern und wiederholst es sofort laut — wie ein Schatten, der dem Sprecher mit nur 1–2 Sekunden Verzögerung folgt. Anders als passives Hören oder Grammatikübungen zwingt Shadowing dein Gehirn und deine Mundmuskulatur, gleichzeitig echte Sprachmuster zu verarbeiten und zu reproduzieren. Studien zeigen, dass es Aussprachegenauigkeit, Intonation, Rhythmus, verbundene Sprache, Hörverständnis und Sprechflüssigkeit signifikant verbessert — was es zu einer der effektivsten Methoden für die IELTS Speaking-Vorbereitung und reale englische Kommunikation macht.

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