Pratique du Shadowing: PCR (Polymerase Chain Reaction) - Apprendre l'anglais à l'oral avec la vidéo

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There are some pieces of technology you really develop an appreciation for.

Maybe it's your phone, your laptop, your drawing tablet, but for me, it is the copy machine.

Oh, the copy machine.

I know it's not possible, but I could promise you that whenever the copy machine was aware of my presence, that's when it decided to malfunction,

especially if it knew I was short on time.

And of course, when it jams, the fancy copy machines will even tell you what is wrong and how to fix it,

but my experience with trying to follow the instructions of fixing the copy machine makes it worse, and then by that point there's a line,

and you feel your heart pounding, and you're embarrassed because now you've jammed it for everyone.

Yeah, so while I've developed an appreciation and respect for the copy machine, I've definitely tried to limit my use of it over the years.

Saves paper anyway.

You may be wondering why is she spending so much time on this?

Well I want to call your attention to a technology, a biotechnology, that works almost like an amazing and fancy copy machine.

Except not for something on paper.

No, it's for DNA.

This biotechnology is PCR.

Polymerase Chain Reaction, or PCR for short.

It provides a way to make more copies of a portion of DNA.

Lots and lots and lots of DNA copies, and this technology does NOT need to happen in a cell either.

In fact, it can happen in a test tube.

And so you may wonder, number one, how does PCR work?

And number two, why?

Why make more copies of some specific portion of DNA?

So let's briefly answer those two questions.

To answer the first question, the how does this work question,

let's talk about what we need first before we get to the steps of how it works.

We need the DNA portion that we want to make copies of, we need some kind of buffer to put it in,

and then we need things that would be necessary to make more copies of the DNA.

So think about what that might mean from our DNA replication video.

We'll need primers.

Recall that primers help DNA polymerase, a building enzyme, know where to go to start its building.

We need DNA polymerase, the building enzyme.

Fun fact, the DNA polymerase used is often a heat -resistant type of DNA polymerase as PCR uses heat.

Typically, the polymerase chosen for the job is tach polymerase, a type of of heat -resistant DNA polymerase.

Tac polymerase is originally from a type of bacteria that can handle and live in really high temperatures in nature, in hot springs.

Pretty cool.

We also need DNA nucleotides for the DNA polymerase to build with.

So we can look at the PCR sequence in three major steps.

We'll illustrate with one double -stranded DNA molecule here, and let's assume this is what we want replicated.

Step 1.

Denaturation.

You've heard that word denature before, right, when we were talking about enzymes?

One way we had talked about denaturing enzymes was using heat.

And heat is what we will use in this step.

This step involves the addition of heat needed to separate the two strands of the DNA molecule.

Step 2.

Annealing.

This word means something a little different in biology.

Basically this is when the two DNA strands

that now have been separated by that heat are going to be cooled and be joined by the primers.

The temperature for this step should allow the primers to bind to the specific segment of DNA that you want to amplify,

which means make copies of.

Step 3.

DNA synthesis.

Remember, synthesis means to make something.

With DNA synthesis, we're going to make more copies of DNA, and to do so, DNA polymerase will begin to work on both of these strands

and it will use the DNA nucleotides as its building material to amplify the DNA.

We should note that the temperature at this step may be a little warmer than the previous step.

It needs to be a temperature that is ideal for the specific DNA polymerase used.

Now after finishing one cycle of this, you have two double -stranded DNA molecules, right?

Similar to how it would be in DNA replication within a cell.

But you can repeat this now, except this time you now have two double -stranded DNA molecules to start with.

So you repeat with the denaturation, annealing, and DNA synthesis steps, now you have four double -stranded DNA molecules.

You repeat the steps again, now you have eight.

And if the process is automated by a machine, which, why yes, they do have in science catalogs, you can actually do this fairly quickly.

Which brings you to, why?

Why do this?

Well, any technology that needs copies of a portion of DNA could find PCR useful, but But we'll just mention two examples in our limited time.

We mentioned DNA fingerprinting in our gel electrophoresis video, and we mentioned that DNA fingerprinting can be part of a crime scene investigation.

Well, in order to have enough copies of DNA samples to run in gel electrophoresis to analyze,

PCR can be performed to make copies of the of the fragments of DNA that are found at a crime scene.

Another example?

Diagnosis of a disease, such as one caused by a virus.

For an especially relevant example, I can mention one of the testing types done for the virus that causes COVID -19.

COVID -19 is a pandemic that we're experiencing right now in 2020.

And the virus that causes COVID -19 is called SARS -CoV -2.

You might have heard of one test type that uses a sample from a nose or throat swab in a PCR test,

but to be more specific, this test for this virus is a real -time reverse transcription PCR test.

The reason it has this fancier reverse transcription in there is

because this virus uses RNA as its genetic material instead of DNA,

and you have to use an enzyme called reverse transcriptase to convert the RNA into DNA.

So before we can do the regular PCR steps we've mentioned, we must convert isolated and purified RNA into DNA.

A specific primer is added that will bind to an area of viral RNA,

and then reverse transcriptase is used to convert viral RNA into cDNA .

Using specific primers and the TAC DNA polymerase,

the cDNA can be copied over and over each cycle in the familiar steps we've been mentioning of PCR.

See the goal is you need enough copies of the viral cDNA in order for it to be detectable.

The idea being if it's a positive result, you have the selective primers binding and you have the TAC DNA polymerase making more

and more copies of the viral cDNA each cycle.

In addition, specific fluorescent probes are also used for identification.

A certain level is needed for identification of a positive result.

And if the virus's genetic material is not present in the sample, then primers wouldn't bind, and there would be no cDNA copies produced.

If you'd like to learn more about this test as far as its limitations

and also its complexity as we're being pretty general here, we've included some further reading suggestions in the video details.

So those were just two examples of how PCR can be used,

but there are many more examples in our suggested further reading links in the video description.

Overall, PCR is such a useful and fascinating technology that will likely remain indispensable for future uses.

It's a rare day when I pull out that word, indispensable.

Well, that's it for the Amoeba Sisters, and we remind you to stay curious.

I'll see you next time.

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Vocabulaire et conseils d’expression pour cette leçon

Les mots qui reviennent le plus souvent : Dna, Pcr, polymerase, step, copies. Cette vidéo contient 83 phrases et 1299 mots à répéter en shadowing. La partie parlée dure 7:30. Le locuteur parle à un rythme naturel d’environ 173 mots par minute, proche d’une conversation courante. Seuls 81 % des mots font partie des 3 000 mots les plus courants en anglais, le vocabulaire est donc exigeant.

Vocabulaire clé de cette vidéo

15 mots de la vidéo à retenir, avec leur prononciation et leur sens :

Mot	Prononciation	Sens
strand nom	/ˈstɹænd/	brin, toron
virus nom	/ˈvaɪ.ɹəs/	virus
enzyme nom	/ˈɛn.zaɪm/	enzyme
molecule nom	/ˈmɑ.lɪ.kjul/	molécule
reverse nom	/ɹɪˈvɝs/	contraire
synthesis nom	/ˈsɪnθəsɪs/	synthèse
convert verbe	/kənˈvɝt/	convertir
genetic adjectif	/d͡ʒəˈnɛt.ɪk/	génétique
identification nom	/aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/	identification
jam nom	/ˈd͡ʒæm/	confiture, marmelade
appreciation nom	/əˌpɹiː.ʃiˈeɪ.ʃən/	appréciation
resistant adjectif	/ɹəˈzɪstənt/	résistant
curious adjectif	/ˈkjʊɹ.i.əs/	curieux
ideal adjectif	/aɪˈdi(ə)l/	idéal
recall verbe	/ɹɪˈkɔl/	rappeler

Les verbes à particule que vous entendrez

Mot	Sens
pull out verbe	se retirer

Des phrases à répéter

Des phrases courtes et complètes de la vidéo, à réutiliser dans la conversation de tous les jours :

You’ve heard that word “denature” before, right?
When we were talking about enzymes?
Which brings you to…why?

La grammaire de cette vidéo

Les structures que le locuteur utilise le plus, avec les mots exacts de la vidéo :

Structure	Dans la vidéo
Voix passive be + participe passé — l’accent est mis sur ce qui arrive, pas sur qui le fait	have been separated · is automated · can be performed
Present perfect have/has + participe passé — une action passée qui compte encore maintenant	you've jammed · I've developed · You've heard

Prononciation à surveiller

Le locuteur utilise 16 contractions et formes réduites, comme we'll, we're, we've. Prononcez-les sous leur forme courte, telles que vous les entendez.

Les sons « th »: synthesis /ˈsɪnθəsɪs/, throat /ˈθɹoʊt/
Les sons « sh » et « zh »: identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, appreciation /əˌpɹiː.ʃiˈeɪ.ʃən/, transcription /tɹænˈskɹɪpʃən/, suggestion /səˈd͡ʒɛst.ʃən/, instruction /ɪnˈstɹʌkʃən/
Mots longs — placez bien l’accent: identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, appreciation /əˌpɹiː.ʃiˈeɪ.ʃən/, biology /baɪˈɑ.lə.d͡ʒi/, complexity /kəmˈplɛk.sɪ.ti/, limitation /lɪmɪˈteɪʃən/

Les sons difficiles pour les francophones :

/tʃ/ et /dʒ/ — à ne pas adoucir en « ch » et « j »: genetic /d͡ʒəˈnɛt.ɪk/, jam /ˈd͡ʒæm/, suggestion /səˈd͡ʒɛst.ʃən/, biology /baɪˈɑ.lə.d͡ʒi/, biotechnology /ˌbaɪ.oʊˌtɛkˈnɑ.lə.d͡ʒi/
/r/ anglais — langue recourbée, sans frotter la gorge: strand /ˈstɹænd/, virus /ˈvaɪ.ɹəs/, reverse /ɹɪˈvɝs/, viral /ˈvaɪɹəl/, appreciation /əˌpɹiː.ʃiˈeɪ.ʃən/

Comment s’entraîner avec cette vidéo

Écoutez la vidéo en entier une fois sans parler et notez les mots que vous ne connaissez pas.
Commencez à la vitesse 0,75×, répétez phrase par phrase, puis revenez à la vitesse normale quand cela devient facile.
Enregistrez-vous et comparez avec l’original, en faisant attention à des mots comme strand, virus, enzyme.

Qu'est-ce que la technique du Shadowing ?

Le Shadowing est une technique d'apprentissage des langues fondée sur la science, développée à l'origine pour la formation des interprètes professionnels. Le principe est simple mais puissant : vous écoutez de l'anglais natif et le répétez immédiatement à voix haute — comme une ombre suivant le locuteur avec un décalage de 1 à 2 secondes. Les recherches montrent une amélioration significative de la précision de la prononciation, de l'intonation, du rythme, des liaisons, de la compréhension orale et de la fluidité.

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Pratique du Shadowing: PCR (Polymerase Chain Reaction) - Apprendre l'anglais à l'oral avec la vidéo

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