Prática de Shadowing: High Resolution Melting (HRM) analysis made easy. - Aprenda a falar inglês com vídeo

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High resolution melting
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or HRM is a very sensitive method to detect sequence variation
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in different samples up to a resolution of a single base pair without the need of sequencing.
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Here you see a wild type of DNA or a PCR product.
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After mutation you may get a homozygous mutant in
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which both the alleles are mutated or a heterozygous mutant in which a single allele is mutated.
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Now you want to check if mutation is there or not.
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HRM is based upon the principle of DNA dissociation with increase in temperature followed by detection by a fluorescent dye.
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The DNA denituration is based on the GC content.
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Higher the GC content, stronger will be the DNA bonds and higher will be the melting temperature to dissociate the two strands.
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In HRM, the detection is assisted by specific binding of a saturating fluorescent dye to the double-stranded DNA
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and measuring its fluorescence in real time on increasing the temperature gradually.
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For HRM, you need to PCR amplify your target gene locus to obtain a specific amplicon.
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Smaller the size of amplicon, better the results will be.
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Then you add a saturating fluorescent dye
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that binds to the dna only in the double stranded form
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then you proceed for hrm analysis in a real time machine here you gradually increase the temperature
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and fluorescence is measured simultaneously the plot shows fluorescence in the y-axis and temperature on the x-axis
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3 different curves are obtained for wild, homozygous and heterozygous mutant.
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Here you see as homozygous mutant had a GC mutation as seen in the previous slide its TM is higher.
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So the curve shifts towards the right.
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Heterozygous mutant on the other hand have a mutation in only one strand.
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So it has a base mismatch and hence the TM is reduced and the curve shifts towards the left.
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method is so sensitive that it detects the variation in the melting temperature up to 0.2 degree Celsius.
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HRM being a simple, economic, rapid and precise method of DNA variant detection, it can be used for mutant identification,
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SNP genotyping, DNA methylation by bisulfide sequencing, DNA fingerprinting and so on.
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This video has been made on a special request by Ms. Sahily Russell.
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Vocabulário e dicas de fala para esta lição

Este vídeo tem 27 frases e 373 palavras para praticar shadowing. A fala dura 2:30. O falante mantém um ritmo estável de cerca de 149 palavras por minuto, confortável para o shadowing. Apenas 66% das palavras estão entre as 3.000 mais comuns do inglês, por isso o vocabulário é exigente.

Vocabulário principal deste vídeo

15 palavras do vídeo que vale a pena aprender, com pronúncia e significado:

PalavraPronúnciaSignificado
mutant substantivo/ˈmju(t)n̩(t)/mutante
strand substantivo/ˈstɹænd/cordel
mutation substantivo/mjuˈteɪʃən/mutação
sequence substantivo/ˈsiː.kwəns/sequência
curve substantivo/kɚv/curva
detection substantivo/dɪˈtɛk.ʃən/detecção, deteção
melt verbo/mɛlt/derreter, fundir
sensitive adjetivo/ˈsɛn.sɪt.ɪv/sensitivo
obtain verbo/əbˈteɪn/obter
gradually advérbio/ˈɡɹæd͡ʒuəli/gradualmente, pouco a pouco
variation substantivo/ˌvɛəɹiˈeɪʃn̩/variação
detect verbo/dɪˈtɛkt/detectar, detetar
axis substantivo/ˈæksɪs/eixo
assist verbo/əˈsɪst/assistir, prestar assistência
hence advérbio/ˈhɛns/daqui

Pronúncia para ficar de olho

  • Os sons de “sh” e “zh”: mutation /mjuˈteɪʃən/, detection /dɪˈtɛk.ʃən/, variation /ˌvɛəɹiˈeɪʃn̩/, identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, dissociate /dɪˈsoʊ.ʃi.eɪt/
  • Palavras longas — acerte a sílaba tônica: identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, simultaneously /ˌsaɪmlˈteɪnɪəsli/, homozygous /ˌhəʊməʊˈzaɪɡəs/, dissociate /dɪˈsoʊ.ʃi.eɪt/, dissociation /dɪˌsoʊsiˈeɪʃən/

Como praticar com este vídeo

  1. Ouça o vídeo inteiro uma vez sem falar e anote as palavras que você não conhece.
  2. Faça shadowing frase por frase na velocidade normal, repetindo cada uma até o seu ritmo ficar igual ao do falante.
  3. Grave a sua voz e compare com o original, prestando atenção a palavras como mutant, strand, mutation.

O que é a Técnica de Shadowing?

Shadowing é uma técnica de aprendizado de idiomas com base científica, originalmente desenvolvida para o treinamento de intérpretes profissionais. O método é simples, mas poderoso: você ouve áudio em inglês nativo e repete imediatamente em voz alta — como uma sombra seguindo o falante com 1-2 segundos de atraso. Pesquisas mostram melhora significativa na precisão da pronúncia, entonação, ritmo, sons conectados, compreensão auditiva e fluência na fala.

Técnica de shadowing: leia o guia completo passo a passo →