Shadowing Practice: High Resolution Melting (HRM) analysis made easy. - Learn English Speaking with Video

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High resolution melting
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or HRM is a very sensitive method to detect sequence variation
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in different samples up to a resolution of a single base pair without the need of sequencing.
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Here you see a wild type of DNA or a PCR product.
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After mutation you may get a homozygous mutant in
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which both the alleles are mutated or a heterozygous mutant in which a single allele is mutated.
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Now you want to check if mutation is there or not.
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HRM is based upon the principle of DNA dissociation with increase in temperature followed by detection by a fluorescent dye.
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The DNA denituration is based on the GC content.
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Higher the GC content, stronger will be the DNA bonds and higher will be the melting temperature to dissociate the two strands.
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In HRM, the detection is assisted by specific binding of a saturating fluorescent dye to the double-stranded DNA
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and measuring its fluorescence in real time on increasing the temperature gradually.
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For HRM, you need to PCR amplify your target gene locus to obtain a specific amplicon.
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Smaller the size of amplicon, better the results will be.
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Then you add a saturating fluorescent dye
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that binds to the dna only in the double stranded form
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then you proceed for hrm analysis in a real time machine here you gradually increase the temperature
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and fluorescence is measured simultaneously the plot shows fluorescence in the y-axis and temperature on the x-axis
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3 different curves are obtained for wild, homozygous and heterozygous mutant.
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Here you see as homozygous mutant had a GC mutation as seen in the previous slide its TM is higher.
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So the curve shifts towards the right.
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Heterozygous mutant on the other hand have a mutation in only one strand.
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So it has a base mismatch and hence the TM is reduced and the curve shifts towards the left.
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method is so sensitive that it detects the variation in the melting temperature up to 0.2 degree Celsius.
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HRM being a simple, economic, rapid and precise method of DNA variant detection, it can be used for mutant identification,
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SNP genotyping, DNA methylation by bisulfide sequencing, DNA fingerprinting and so on.
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This video has been made on a special request by Ms. Sahily Russell.
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Vocabulario y notas de pronunciación para esta lección

Este vídeo tiene 27 frases y 373 palabras para practicar shadowing. La parte hablada dura 2:30. El hablante mantiene un ritmo constante de unas 149 palabras por minuto, cómodo para el shadowing. Solo el 66 % de las palabras está entre las 3.000 más comunes del inglés, por lo que el vocabulario es exigente.

Vocabulario clave de este vídeo

15 palabras del vídeo que vale la pena aprender, con su pronunciación y significado:

PalabraPronunciaciónSignificado
mutant sustantivo/ˈmju(t)n̩(t)/mutante
strand sustantivo/ˈstɹænd/hebra, filástica
mutation sustantivo/mjuˈteɪʃən/mutación
sequence sustantivo/ˈsiː.kwəns/secuencia
curve sustantivo/kɚv/curva
detection sustantivo/dɪˈtɛk.ʃən/detección
melt verbo/mɛlt/derretirse, fundirse
sensitive adjetivo/ˈsɛn.sɪt.ɪv/sensible
obtain verbo/əbˈteɪn/obtener
gradually adverbio/ˈɡɹæd͡ʒuəli/gradualmente, paulatinamente
variation sustantivo/ˌvɛəɹiˈeɪʃn̩/variación
detect verbo/dɪˈtɛkt/detectar
axis sustantivo/ˈæksɪs/eje
assist verbo/əˈsɪst/ayudar, asistir
hence adverbio/ˈhɛns/de aquí/acá/aquende

Pronunciación a tener en cuenta

  • Los sonidos de “sh” y “zh”: mutation /mjuˈteɪʃən/, detection /dɪˈtɛk.ʃən/, variation /ˌvɛəɹiˈeɪʃn̩/, identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, dissociate /dɪˈsoʊ.ʃi.eɪt/
  • Palabras largas — cuida el acento: identification /aɪˌdɛn(t)ɪfɪˈkeɪʃn̩/, simultaneously /ˌsaɪmlˈteɪnɪəsli/, homozygous /ˌhəʊməʊˈzaɪɡəs/, dissociate /dɪˈsoʊ.ʃi.eɪt/, dissociation /dɪˌsoʊsiˈeɪʃən/

Cómo practicar con este vídeo

  1. Escucha el vídeo entero una vez sin hablar y anota las palabras que no conoces.
  2. Haz shadowing frase por frase a velocidad normal, repitiendo cada una hasta que tu ritmo coincida con el del hablante.
  3. Grábate y compara con el original, prestando atención a palabras como mutant, strand, mutation.

¿Qué es la Técnica de Shadowing?

Shadowing es una técnica de aprendizaje de idiomas respaldada por la ciencia, desarrollada originalmente para la formación de intérpretes profesionales y popularizada por el políglota Dr. Alexander Arguelles. El método es simple pero poderoso: escuchas audio en inglés nativo y lo repites en voz alta de inmediato, como una sombra que sigue al hablante con solo 1-2 segundos de retraso. A diferencia de la escucha pasiva o los ejercicios de gramática, el shadowing obliga a tu cerebro y músculos de la boca a procesar y reproducir simultáneamente patrones de habla reales. Las investigaciones muestran que mejora significativamente la precisión de la pronunciación, la entonación, el ritmo, el habla conectada, la comprensión auditiva y la fluidez al hablar, convirtiéndola en una de las metodologías más efectivas para la preparación del IELTS Speaking y la comunicación en inglés en el mundo real.

Técnica de shadowing: lee la guía completa paso a paso →